Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus).
Identifieur interne : 003274 ( Main/Exploration ); précédent : 003273; suivant : 003275Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus).
Auteurs : Zhichun Jia [République populaire de Chine] ; Jiqing Gou ; Yimin Sun ; Li Yuan ; Qiao Tang ; Xingyong Yang ; Yan Pei ; Keming LuoSource :
- Tree physiology [ 0829-318X ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Leonurus, Maladies des plantes, Populus.
- immunologie : Maladies des plantes.
- microbiologie : Feuilles de plante, Maladies des plantes.
- métabolisme : Populus.
- Prédisposition génétique à une maladie, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- genetics : Leonurus, Plant Diseases, Populus.
- immunology : Plant Diseases.
- metabolism : Populus.
- microbiology : Plant Diseases, Plant Leaves.
- Genetic Predisposition to Disease, Plants, Genetically Modified.
Abstract
The antimicrobial protein gene LJAMP2 is a plant non-specific lipid transfer protein from motherwort (Leonurus japonicus). In this study, it was introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation with neomycin phosphotransferase II gene conferring kanamycin resistance as selectable marker. A total of 16 poplar lines were obtained, and polymerase chain reaction (PCR) analysis established the stable integration of transgenes in the plant genome. Reverse transcription-PCR detected LJAMP2 expression in transgenic plants. Resistance to fungal pathogens Alternaria alternata (Fr.) Keissler and Colletotrichum gloeosporioides (Penz.) of transgenic poplar lines was tested. In vitro inhibitory activity against the fungal pathogens was evident from the crude leaf extracts from the transformants. In vivo assays showed that, after infection with both A. alternata (Fr.) Keissler and C. gloeosporioides (Penz.), there was a significant reduction in disease symptoms in transgenic poplar plants compared with the control. These results suggest that constitutive expression of the LJAMP2 gene from motherwort can be exploited to improve resistance to fungal pathogens in poplar.
DOI: 10.1093/treephys/tpq093
PubMed: 21084346
Affiliations:
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Jia</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Eco-environment and Bio-resource of Three Gorges Reservoir Region, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Eco-environment and Bio-resource of Three Gorges Reservoir Region, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715</wicri:regionArea><wicri:noRegion>Chongqing 400715</wicri:noRegion></affiliation></author><author><name sortKey="Gou, Jiqing" sort="Gou, Jiqing" uniqKey="Gou J" first="Jiqing" last="Gou">Jiqing Gou</name></author><author><name sortKey="Sun, Yimin" sort="Sun, Yimin" uniqKey="Sun Y" first="Yimin" last="Sun">Yimin Sun</name></author><author><name sortKey="Yuan, Li" sort="Yuan, Li" uniqKey="Yuan L" first="Li" last="Yuan">Li Yuan</name></author><author><name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name></author><author><name sortKey="Yang, Xingyong" sort="Yang, Xingyong" uniqKey="Yang X" first="Xingyong" last="Yang">Xingyong Yang</name></author><author><name sortKey="Pei, Yan" sort="Pei, Yan" uniqKey="Pei Y" first="Yan" last="Pei">Yan Pei</name></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Genetic Predisposition to Disease (MeSH)</term><term>Leonurus (genetics)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (microbiologie)</term><term>Leonurus (génétique)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (immunologie)</term><term>Maladies des plantes (microbiologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Prédisposition génétique à une maladie (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Leonurus</term><term>Plant Diseases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Leonurus</term><term>Maladies des plantes</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Predisposition to Disease</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Prédisposition génétique à une maladie</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The antimicrobial protein gene LJAMP2 is a plant non-specific lipid transfer protein from motherwort (Leonurus japonicus). In this study, it was introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation with neomycin phosphotransferase II gene conferring kanamycin resistance as selectable marker. A total of 16 poplar lines were obtained, and polymerase chain reaction (PCR) analysis established the stable integration of transgenes in the plant genome. Reverse transcription-PCR detected LJAMP2 expression in transgenic plants. Resistance to fungal pathogens Alternaria alternata (Fr.) Keissler and Colletotrichum gloeosporioides (Penz.) of transgenic poplar lines was tested. In vitro inhibitory activity against the fungal pathogens was evident from the crude leaf extracts from the transformants. In vivo assays showed that, after infection with both A. alternata (Fr.) Keissler and C. gloeosporioides (Penz.), there was a significant reduction in disease symptoms in transgenic poplar plants compared with the control. These results suggest that constitutive expression of the LJAMP2 gene from motherwort can be exploited to improve resistance to fungal pathogens in poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21084346</PMID><DateCompleted><Year>2011</Year><Month>03</Month><Day>08</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>30</Volume><Issue>12</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus).</ArticleTitle><Pagination><MedlinePgn>1599-605</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpq093</ELocationID><Abstract><AbstractText>The antimicrobial protein gene LJAMP2 is a plant non-specific lipid transfer protein from motherwort (Leonurus japonicus). In this study, it was introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation with neomycin phosphotransferase II gene conferring kanamycin resistance as selectable marker. A total of 16 poplar lines were obtained, and polymerase chain reaction (PCR) analysis established the stable integration of transgenes in the plant genome. Reverse transcription-PCR detected LJAMP2 expression in transgenic plants. Resistance to fungal pathogens Alternaria alternata (Fr.) Keissler and Colletotrichum gloeosporioides (Penz.) of transgenic poplar lines was tested. In vitro inhibitory activity against the fungal pathogens was evident from the crude leaf extracts from the transformants. In vivo assays showed that, after infection with both A. alternata (Fr.) Keissler and C. gloeosporioides (Penz.), there was a significant reduction in disease symptoms in transgenic poplar plants compared with the control. These results suggest that constitutive expression of the LJAMP2 gene from motherwort can be exploited to improve resistance to fungal pathogens in poplar.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Zhichun</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Eco-environment and Bio-resource of Three Gorges Reservoir Region, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gou</LastName><ForeName>Jiqing</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Yimin</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Qiao</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xingyong</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Pei</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Keming</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>11</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020022" MajorTopicYN="N">Genetic Predisposition to Disease</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031332" MajorTopicYN="N">Leonurus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>11</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>11</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>3</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21084346</ArticleId><ArticleId IdType="pii">tpq093</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpq093</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Gou, Jiqing" sort="Gou, Jiqing" uniqKey="Gou J" first="Jiqing" last="Gou">Jiqing Gou</name><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name><name sortKey="Pei, Yan" sort="Pei, Yan" uniqKey="Pei Y" first="Yan" last="Pei">Yan Pei</name><name sortKey="Sun, Yimin" sort="Sun, Yimin" uniqKey="Sun Y" first="Yimin" last="Sun">Yimin Sun</name><name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name><name sortKey="Yang, Xingyong" sort="Yang, Xingyong" uniqKey="Yang X" first="Xingyong" last="Yang">Xingyong Yang</name><name sortKey="Yuan, Li" sort="Yuan, Li" uniqKey="Yuan L" first="Li" last="Yuan">Li Yuan</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Jia, Zhichun" sort="Jia, Zhichun" uniqKey="Jia Z" first="Zhichun" last="Jia">Zhichun Jia</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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